Large boulders have been installed along sections of the track at the popular Edi Cutting campground in the King Valley, preventing vehicular access to track shoulders and a number of riverside sites.
The works were carried about by Forest Fire Management Victoria, which stated it was "to improve visitor safety and access."
A Forest Fire Management Victoria spokesperson said the works aim to better define designated camping and day visitor areas.
They said it was undertaken following visitor feedback.
"This will help ensure that visitors do not camp over the access track and will help protect the King River frontage," the spokesperson said.
"The day visitor area will also ensure that all visitors can enjoy the site and access the popular ‘beach’ swimming area."
Edi Cutting Reserve is a free bush camping area which runs for about two kilometres alongside the King River, with several fire pits and two pit toilets – one located at each end of the camp.
Forest Fire Management Victoria says it attracts about 40,000 campers and day visitors each year.
Sites cannot be booked, and are available on a first-come-first-served basis, and locals say the campground's popularity has grown exponentially in recent years, with thousands now accessing the site year-round; some camping on road and riversides during busy holiday periods.
Forest Fire Management Victoria said signage is to be erected at the Edi Cutting campground in coming weeks which will outline the camping and day visitor guidelines.